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pi16 rabbit us biological  (R&D Systems)
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1 ap  (Proteintech)
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1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pecam1 (cd31) rat (clone 390)  (Millipore)
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Pecam1 (Cd31) Rat (Clone 390), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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myh11 rabbit proteintech 21404 1 ap  (Proteintech)
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Figure 5. Distribution of PDGFRAþ fibroblasts relative to other tissue types, cells, and markers in the bladder wall. A–E: representative confocal micro- graphs showing the distribution of PDGFRA þ fibroblasts with respect to KRT5- and KRT20-labeled urothelium (A), PECAM1-labeled endothelial cells (B), <t>MYH11-labeled</t> smooth muscle cells (C), PTPRC-labeled immune cells (D), or UCHL1-labeled nerve fibers (E). Note that the urothelium (Ut; umbrella cells in particular) was also UCHL1 þ. The dashed boxed regions in the left images are magnified in the middle and right images. Representative confocal micrographs from 46 mice are shown. F and G: nuclei in confocal Z-stacks were detected, and the mean fluorescent intensity of the indicated markers associated with each nucleus was measured and plotted using Volumetric Tissue Exploration and Analysis tissue cytometry software. Each dot repre- sents a single nucleus and its associated mean intensity for the indicated markers. The data were gated into quadrants with quadrant 1 representing cells that were just PDGFRA þ, quadrant 2 representing cells that were gated as positive for both markers, quadrant 3 representing cells that were “neg- ative” for both markers, and quadrant 4 representing cells positive for just the indicated marker. H: the colocalization coefficient for cells positive for both PDGFRA and the indicated marker, or vice versa, was calculated and plotted. Analysis was performed on random sections taken from the bladders of 4 animals. Data are means ± SD.
Myh11 Rabbit Proteintech 21404 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stock acta2 rabbit proteintech 14395 1 ap  (Proteintech)
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Figure 9. PDGFRAþ suburothelial fibroblasts express <t>ACTA2,</t> CAR3, and TNC. A: ACTA2 was expressed by smooth muscle cells (SMC) in the muscularis externa and blood vessels (BV) within the lamina propria. A weaker, but reproducible, ACTA2 signal was also observed in suburo- thelial fibroblasts, subjacent to the urothelium (Ut). The dashed boxed region in the top right image is magnified in the bottom images. Nuclei of PDGFRA þ;ACTA2 þ cells are indicated by yellow arrows. B: expression of TNC and CAR3 by PDGFRAþ suburothelial fibroblasts. The dashed boxed region in the right image is reproduced in the left images. Cells with visible nuclei expressing all three markers are indicated by white arrows. Note that PDGFRA is a mem- brane protein, CAR3 is a cytoplasmic protein, and TNC is a secreted protein that is deposited in the space between ad- jacent fibroblasts. All images are confocal micrographs and representative of images taken from three-five separate experiments each with one-two mice.
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Image Search Results


Figure 5. Distribution of PDGFRAþ fibroblasts relative to other tissue types, cells, and markers in the bladder wall. A–E: representative confocal micro- graphs showing the distribution of PDGFRA þ fibroblasts with respect to KRT5- and KRT20-labeled urothelium (A), PECAM1-labeled endothelial cells (B), MYH11-labeled smooth muscle cells (C), PTPRC-labeled immune cells (D), or UCHL1-labeled nerve fibers (E). Note that the urothelium (Ut; umbrella cells in particular) was also UCHL1 þ. The dashed boxed regions in the left images are magnified in the middle and right images. Representative confocal micrographs from 46 mice are shown. F and G: nuclei in confocal Z-stacks were detected, and the mean fluorescent intensity of the indicated markers associated with each nucleus was measured and plotted using Volumetric Tissue Exploration and Analysis tissue cytometry software. Each dot repre- sents a single nucleus and its associated mean intensity for the indicated markers. The data were gated into quadrants with quadrant 1 representing cells that were just PDGFRA þ, quadrant 2 representing cells that were gated as positive for both markers, quadrant 3 representing cells that were “neg- ative” for both markers, and quadrant 4 representing cells positive for just the indicated marker. H: the colocalization coefficient for cells positive for both PDGFRA and the indicated marker, or vice versa, was calculated and plotted. Analysis was performed on random sections taken from the bladders of 4 animals. Data are means ± SD.

Journal: American journal of physiology. Renal physiology

Article Title: Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA + interstitial cells are fibroblasts.

doi: 10.1152/ajprenal.00135.2022

Figure Lengend Snippet: Figure 5. Distribution of PDGFRAþ fibroblasts relative to other tissue types, cells, and markers in the bladder wall. A–E: representative confocal micro- graphs showing the distribution of PDGFRA þ fibroblasts with respect to KRT5- and KRT20-labeled urothelium (A), PECAM1-labeled endothelial cells (B), MYH11-labeled smooth muscle cells (C), PTPRC-labeled immune cells (D), or UCHL1-labeled nerve fibers (E). Note that the urothelium (Ut; umbrella cells in particular) was also UCHL1 þ. The dashed boxed regions in the left images are magnified in the middle and right images. Representative confocal micrographs from 46 mice are shown. F and G: nuclei in confocal Z-stacks were detected, and the mean fluorescent intensity of the indicated markers associated with each nucleus was measured and plotted using Volumetric Tissue Exploration and Analysis tissue cytometry software. Each dot repre- sents a single nucleus and its associated mean intensity for the indicated markers. The data were gated into quadrants with quadrant 1 representing cells that were just PDGFRA þ, quadrant 2 representing cells that were gated as positive for both markers, quadrant 3 representing cells that were “neg- ative” for both markers, and quadrant 4 representing cells positive for just the indicated marker. H: the colocalization coefficient for cells positive for both PDGFRA and the indicated marker, or vice versa, was calculated and plotted. Analysis was performed on random sections taken from the bladders of 4 animals. Data are means ± SD.

Article Snippet: Primary antibodies used in the present study Antigen Host Species Source Catalog Number Dilution of stock ACTA2 Rabbit Proteintech 14395-1-AP 1:100 CAR3 Rabbit Proteintech 15197-1-AP 1:100 (1:100 for FISHID) CD34 Sheep R&D Systems AF6518 1:100 (1:50 for FISHID) CD34 Rat (clone MEC14.7) Invitrogen (ThermoFisher) MA1-22646 1:200 CSPG4 Rabbit Millipore Sigma AB5320 1:100 GFP Rabbit Abcam Ab6556 1:75 for EM KIT (CD117) Rat (clone ACK2) Stemcell 60034.1 1:100 KRT20 Rabbit Abcam ab53120 1:100 KRT5 Chicken BioLegend 905901 1:400 LY6A Rat BioLegend 108101 1:100 MYH11 Rabbit Proteintech 21404-1-AP 1:100 (1:100 for FISHID) PDGFRA Goat R&D Systems AF1062 1:100 (1:50 for FISHID) Preferred antibody used in most experiments PECAM1 (CD31) Rat (clone 390) Millipore Sigma CBL1337-I 1:100 PI16 Goat R&D Systems AF4929-SP 1:100 PI16 Rabbit US Biological 141796 1:100 PTPRC (CD45) Rat (clone IBL-3/16) Bio-Rad MCA1388 1:100 TNC Rat (clone no. 578) R&D Systems MAB2138 1:400 UCHL1 (PGP9.5) Rabbit Genetex GTX109637 1:100 VIM Rat BioLegend 699301 1:100 FISHID, fluorescent in situ hybridization with immunodetection.

Techniques: Labeling, Cytometry, Software, Marker

Figure 6. PDGFRAþ cells express Col1a2. A: expression of Col1a2-driven mG (mem- brane-bound enhanced green fluorescent protein) in tamoxifen-treated experimental Col1a2Cre-ERT þ /–;RosamT/mG þ /– or control Col1a2Cre-ERT–/–;RosamT/mG þ /– mice. B: coexpression of mG in the PDGFRAþ, CAR3 þ, or CD34þ cells of the indicated regions of the bladder wall [suburothelial (SU), outer lamina propria (OLP), intermus- cular (IM), and serosal (Se)]. Examples of cells with visible nuclei coexpressing these markers are marked with yellow arrows. Coexpression of MYH11 and mG was also assessed in the bottom right images. Examples of smooth muscle cells expressing mG are indicated by white arrowheads. C: use of fluorescent in situ hybridization with immunodetection (FISHID) to assess coexpression of PDGFRA, Pdgfra, and Col1a2 in the indicated regions of the bladder wall. Examples of cells (i.e., nuclei) that expressed all three markers are labeled with white arrowheads; cells that were PDGFRAþ;Pdgfraþ are marked with green arrowheads. The image marked “Control” was performed in the absence of anti- PDGFRA antibody and using the three-plex negative control probe against Bacillus sub- tilis dapB. All images are confocal micro- graphs and representative of images taken from two separate experiments each using two mice. BV, blood vessels; MC, mesothe- lial cells; Ut, urothelium.

Journal: American journal of physiology. Renal physiology

Article Title: Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA + interstitial cells are fibroblasts.

doi: 10.1152/ajprenal.00135.2022

Figure Lengend Snippet: Figure 6. PDGFRAþ cells express Col1a2. A: expression of Col1a2-driven mG (mem- brane-bound enhanced green fluorescent protein) in tamoxifen-treated experimental Col1a2Cre-ERT þ /–;RosamT/mG þ /– or control Col1a2Cre-ERT–/–;RosamT/mG þ /– mice. B: coexpression of mG in the PDGFRAþ, CAR3 þ, or CD34þ cells of the indicated regions of the bladder wall [suburothelial (SU), outer lamina propria (OLP), intermus- cular (IM), and serosal (Se)]. Examples of cells with visible nuclei coexpressing these markers are marked with yellow arrows. Coexpression of MYH11 and mG was also assessed in the bottom right images. Examples of smooth muscle cells expressing mG are indicated by white arrowheads. C: use of fluorescent in situ hybridization with immunodetection (FISHID) to assess coexpression of PDGFRA, Pdgfra, and Col1a2 in the indicated regions of the bladder wall. Examples of cells (i.e., nuclei) that expressed all three markers are labeled with white arrowheads; cells that were PDGFRAþ;Pdgfraþ are marked with green arrowheads. The image marked “Control” was performed in the absence of anti- PDGFRA antibody and using the three-plex negative control probe against Bacillus sub- tilis dapB. All images are confocal micro- graphs and representative of images taken from two separate experiments each using two mice. BV, blood vessels; MC, mesothe- lial cells; Ut, urothelium.

Article Snippet: Primary antibodies used in the present study Antigen Host Species Source Catalog Number Dilution of stock ACTA2 Rabbit Proteintech 14395-1-AP 1:100 CAR3 Rabbit Proteintech 15197-1-AP 1:100 (1:100 for FISHID) CD34 Sheep R&D Systems AF6518 1:100 (1:50 for FISHID) CD34 Rat (clone MEC14.7) Invitrogen (ThermoFisher) MA1-22646 1:200 CSPG4 Rabbit Millipore Sigma AB5320 1:100 GFP Rabbit Abcam Ab6556 1:75 for EM KIT (CD117) Rat (clone ACK2) Stemcell 60034.1 1:100 KRT20 Rabbit Abcam ab53120 1:100 KRT5 Chicken BioLegend 905901 1:400 LY6A Rat BioLegend 108101 1:100 MYH11 Rabbit Proteintech 21404-1-AP 1:100 (1:100 for FISHID) PDGFRA Goat R&D Systems AF1062 1:100 (1:50 for FISHID) Preferred antibody used in most experiments PECAM1 (CD31) Rat (clone 390) Millipore Sigma CBL1337-I 1:100 PI16 Goat R&D Systems AF4929-SP 1:100 PI16 Rabbit US Biological 141796 1:100 PTPRC (CD45) Rat (clone IBL-3/16) Bio-Rad MCA1388 1:100 TNC Rat (clone no. 578) R&D Systems MAB2138 1:400 UCHL1 (PGP9.5) Rabbit Genetex GTX109637 1:100 VIM Rat BioLegend 699301 1:100 FISHID, fluorescent in situ hybridization with immunodetection.

Techniques: Expressing, Control, In Situ Hybridization, Immunodetection, Labeling, Negative Control

Figure 9. PDGFRAþ suburothelial fibroblasts express ACTA2, CAR3, and TNC. A: ACTA2 was expressed by smooth muscle cells (SMC) in the muscularis externa and blood vessels (BV) within the lamina propria. A weaker, but reproducible, ACTA2 signal was also observed in suburo- thelial fibroblasts, subjacent to the urothelium (Ut). The dashed boxed region in the top right image is magnified in the bottom images. Nuclei of PDGFRA þ;ACTA2 þ cells are indicated by yellow arrows. B: expression of TNC and CAR3 by PDGFRAþ suburothelial fibroblasts. The dashed boxed region in the right image is reproduced in the left images. Cells with visible nuclei expressing all three markers are indicated by white arrows. Note that PDGFRA is a mem- brane protein, CAR3 is a cytoplasmic protein, and TNC is a secreted protein that is deposited in the space between ad- jacent fibroblasts. All images are confocal micrographs and representative of images taken from three-five separate experiments each with one-two mice.

Journal: American journal of physiology. Renal physiology

Article Title: Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA + interstitial cells are fibroblasts.

doi: 10.1152/ajprenal.00135.2022

Figure Lengend Snippet: Figure 9. PDGFRAþ suburothelial fibroblasts express ACTA2, CAR3, and TNC. A: ACTA2 was expressed by smooth muscle cells (SMC) in the muscularis externa and blood vessels (BV) within the lamina propria. A weaker, but reproducible, ACTA2 signal was also observed in suburo- thelial fibroblasts, subjacent to the urothelium (Ut). The dashed boxed region in the top right image is magnified in the bottom images. Nuclei of PDGFRA þ;ACTA2 þ cells are indicated by yellow arrows. B: expression of TNC and CAR3 by PDGFRAþ suburothelial fibroblasts. The dashed boxed region in the right image is reproduced in the left images. Cells with visible nuclei expressing all three markers are indicated by white arrows. Note that PDGFRA is a mem- brane protein, CAR3 is a cytoplasmic protein, and TNC is a secreted protein that is deposited in the space between ad- jacent fibroblasts. All images are confocal micrographs and representative of images taken from three-five separate experiments each with one-two mice.

Article Snippet: Primary antibodies used in the present study Antigen Host Species Source Catalog Number Dilution of stock ACTA2 Rabbit Proteintech 14395-1-AP 1:100 CAR3 Rabbit Proteintech 15197-1-AP 1:100 (1:100 for FISHID) CD34 Sheep R&D Systems AF6518 1:100 (1:50 for FISHID) CD34 Rat (clone MEC14.7) Invitrogen (ThermoFisher) MA1-22646 1:200 CSPG4 Rabbit Millipore Sigma AB5320 1:100 GFP Rabbit Abcam Ab6556 1:75 for EM KIT (CD117) Rat (clone ACK2) Stemcell 60034.1 1:100 KRT20 Rabbit Abcam ab53120 1:100 KRT5 Chicken BioLegend 905901 1:400 LY6A Rat BioLegend 108101 1:100 MYH11 Rabbit Proteintech 21404-1-AP 1:100 (1:100 for FISHID) PDGFRA Goat R&D Systems AF1062 1:100 (1:50 for FISHID) Preferred antibody used in most experiments PECAM1 (CD31) Rat (clone 390) Millipore Sigma CBL1337-I 1:100 PI16 Goat R&D Systems AF4929-SP 1:100 PI16 Rabbit US Biological 141796 1:100 PTPRC (CD45) Rat (clone IBL-3/16) Bio-Rad MCA1388 1:100 TNC Rat (clone no. 578) R&D Systems MAB2138 1:400 UCHL1 (PGP9.5) Rabbit Genetex GTX109637 1:100 VIM Rat BioLegend 699301 1:100 FISHID, fluorescent in situ hybridization with immunodetection.

Techniques: Expressing